BODIPY-FL

BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm[1][2][3][4].

Product Specifications

CAS Number

[165599-63-3]

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/bodipy-fl.html

Purity

99.98

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])O

Molecular Formula

C14H15BF2N2O2

Molecular Weight

292.09

Precautions

H302, H315, H319, H335

References & Citations

[1]Leung K. BODIPY-FL-neutravidin-biotin-Cetuximab. 2008 Jan 15 [updated 2008 Feb 25]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD) : National Center for Biotechnology Information (US) ; 2004–2013. PMID: 20641313.|[2]Kurata S, et al. Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY ((R) ) FL-labeled probe or primer. Nucleic Acids Res. 2001 Mar 15;29 (6) :E34. |[3]Fan W, et al. Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells. Bio Protoc. 2022 Nov 20;12 (22) :e4555.|[4]Guseva GB, et al. Design, Spectral Characteristics, and Possibilities for Practical Application of BODIPY FL-Labeled Monoterpenoid. ACS Appl Bio Mater. 2021 Aug 16;4 (8) :6227-6235.