Rabbit anti-USP25 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to USP25
Synonyms
USP21; Ubiquitin carboxyl-terminal hydrolase 25; Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human USP25
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of USP25 expression in SKOV3 (A), PC12 (B), mouse brain (C), mouse liver (D) whole cell lysates.
IHC description
Immunohistochemical analysis of USP25 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
29761
Gene ID (Mouse)
30940
SwissProt (Human)
Q9UHP3
SwissProt (Mouse)
P57080
CAS Number
9007-83-4
Available Sizes
Curated Selection
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