Rabbit anti-RPS6KL1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RPS6KL1
Synonyms
Ribosomal protein S6 kinase-like 1
Host
Rabbit
Reactivity
H, M
Immunogen
Recombinant full length protein of human RPS6KL1
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of RPS6KL1 expression in THP1 (A), mouse brain (B), mouse heart (C) whole cell lysates.
IHC description
Immunohistochemical analysis of RPS6KL1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of RPS6KL1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
83694
Gene ID (Mouse)
238323
SwissProt (Human)
Q9Y6S9
SwissProt (Mouse)
Q8R2S1
CAS Number
9007-83-4
Available Sizes
Curated Selection
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