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Rabbit anti-PSMA5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PSMA5

Synonyms

Proteasome subunit alpha type-5; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PSMA5

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PSMA5 expression in MCF7 (A), HepG2 (B), mouse liver (C), mouse heart (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PSMA5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5686

Gene ID (Mouse)

26442

Gene ID (Rat)

29672

SwissProt (Human)

P28066

SwissProt (Mouse)

Q9Z2U1

SwissProt (Rat)

P34064

CAS Number

9007-83-4

Available Sizes

Curated Selection

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