Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-p53 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to p53

Synonyms

P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Host

Rabbit

Reactivity

H, Mk, P, RB

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p53. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of p53 expression in Jurkat (A), HEK293T (B), COS7 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of p53 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of p53 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

7157

SwissProt (Human)

P04637

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Rabbit Anti ARFGAP2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA) 120ul
IRBAMLARFGAP2AP647120UL 120 µL

Rabbit Anti ARFGAP2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA) 120ul

Ask
View Details
AGAP1 Protein Vector (Human) (pPM-C-HA)
11492021 500 ng

AGAP1 Protein Vector (Human) (pPM-C-HA)

Ask
View Details
SPINK8 CRISPR Activation Plasmid (h)
sc-418693-ACT 20 µg

SPINK8 CRISPR Activation Plasmid (h)

Ask
View Details
Human ITGAV & ITGB1 Heterodimer Protein, His Tag & hFc Tag
TP724068 100 µg

Human ITGAV & ITGB1 Heterodimer Protein, His Tag & hFc Tag

Ask
View Details