Rabbit anti-p47 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to p47
Synonyms
UBXN2C; NSFL1 cofactor p47; UBX domain-containing protein 2C; p97 cofactor p47
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human p47
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of p47 expression in mouse brain (A), rat kidney (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of p47 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
55968
Gene ID (Mouse)
386649
Gene ID (Rat)
83809
SwissProt (Human)
Q9UNZ2
SwissProt (Mouse)
Q9CZ44
SwissProt (Rat)
O35987
CAS Number
9007-83-4
Available Sizes
Curated Selection
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