Rabbit anti-MARCH1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to MARCH1
Synonyms
RNF171; E3 ubiquitin-protein ligase MARCH1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; MARCH-I; RING finger protein 171
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARCH1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of MARCH1 expression in K562 (A), mouse brain (B) whole cell lysates.
IHC description
Immunohistochemical analysis of MARCH1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
55016
Gene ID (Mouse)
72925
SwissProt (Human)
Q8TCQ1
SwissProt (Mouse)
Q6NZQ8
CAS Number
9007-83-4
Available Sizes
Curated Selection
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