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Rabbit anti-Granzyme B/H Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Granzyme B/H

Synonyms

GZMB; CGL1; CSPB; CTLA1; GRB; Granzyme B; C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E; GZMH; CGL2; CTSGL2; Granzyme H; CCP-X; Cathepsin G-like 2; CTSGL2; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C; CSP-C

Host

Rabbit

Reactivity

H, M, R, B, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Granzyme B/H. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Granzyme B/H expression in MCF7 (A), Raw264.7 (B), SP2/0 (C), H9C2 (D), HL60 (E) whole cell lysates.

IHC description

Immunohistochemical analysis of Granzyme B/H staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

2999

Gene ID (Mouse)

14939

Gene ID (Rat)

100361468

SwissProt (Human)

P10144; P20718

SwissProt (Mouse)

P04187

SwissProt (Rat)

P18291

CAS Number

9007-83-4

Available Sizes

Curated Selection

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