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Rabbit anti-GPR48 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GPR48

Synonyms

GPR48; Leucine-rich repeat-containing G-protein coupled receptor 4; G-protein coupled receptor 48

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR48. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GPR48 expression in HEK293T (A), PC3 (B), SP20 (C), PC12 (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of GPR48 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

55366

Gene ID (Mouse)

107515

Gene ID (Rat)

286994

SwissProt (Human)

Q9BXB1

SwissProt (Mouse)

A2ARI4

SwissProt (Rat)

Q9Z2H4

CAS Number

9007-83-4

Available Sizes

Curated Selection

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