Rabbit anti-GFRA1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GFRA1
Synonyms
GDNFRA; RETL1; TRNR1; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human GFRA1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GFRA1 expression in MCF7 (A), A549 (B), SW480 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of GFRA1 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2674
Gene ID (Mouse)
14585
Gene ID (Rat)
25454
SwissProt (Human)
P56159
SwissProt (Mouse)
P97785
SwissProt (Rat)
Q62997
CAS Number
9007-83-4
Available Sizes
Curated Selection
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