Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-Focal Adhesion Kinase Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Focal Adhesion Kinase

Synonyms

FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

Host

Rabbit

Reactivity

H, M

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Focal Adhesion Kinase. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of Focal Adhesion Kinase expression in HT29 (A), Hela (B), NIH3T3 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Focal Adhesion Kinase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Focal Adhesion Kinase staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

5747

Gene ID (Mouse)

14083

SwissProt (Human)

Q05397

SwissProt (Mouse)

P34152

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

KRT35 Antibody
OACA05874-50UG 50 µg

KRT35 Antibody

Ask
View Details
PYGL Polyclonal Antibody, Biotin Conjugated
bs-5011R-Biotin 100 µL

PYGL Polyclonal Antibody, Biotin Conjugated

Ask
View Details
Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit
MBS7253259-01 48 Well

Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit

Ask
View Details
Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit
MBS7253259-02 96 Well

Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit

Ask
View Details
Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit
MBS7253259-03 5x 96 Well

Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit

Ask
View Details
Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit
MBS7253259-04 10x 96 Well

Human Arf GAP with GTPase, ANK repeat and PH domain containing protein 8 (AGAP8) ELISA Kit

Ask
View Details