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Rabbit anti-EIF2S1 (pS51) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to EIF2S1 (pS51)

Synonyms

EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha

Host

Rabbit

Reactivity

H, M, R, B, C, P, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF2S1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of EIF2S1 (pS51) expression in HeLa (A), PC12 (B), A431 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of EIF2S1 (pS51) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1965

Gene ID (Mouse)

13665

Gene ID (Rat)

54318

SwissProt (Human)

P05198

SwissProt (Mouse)

Q6ZWX6

SwissProt (Rat)

P68101

CAS Number

9007-83-4

Available Sizes

Curated Selection

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