Rabbit anti-DHFR Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DHFR
Synonyms
Dihydrofolate reductase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human DHFR
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of DHFR expression in Hela (A), HL60 (B), THP1 (C), mouse liver (D) whole cell lysates.
IHC description
Immunohistochemical analysis of DHFR staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1719
Gene ID (Mouse)
13361
Gene ID (Rat)
24312
SwissProt (Human)
P00374
SwissProt (Mouse)
P00375
SwissProt (Rat)
Q920D2
CAS Number
9007-83-4
Available Sizes
Curated Selection
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