Rabbit anti-Cyclophilin D Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Cyclophilin D
Synonyms
CYP40; CYPD; Peptidyl-prolyl cis-trans isomerase D; PPIase D; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Cyclophilin D
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Cyclophilin D expression in HepG2 (A), NIH3T3 (B), mouse brain (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Cyclophilin D staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5481
Gene ID (Mouse)
67738
Gene ID (Rat)
361967
SwissProt (Human)
Q08752
SwissProt (Mouse)
Q9CR16
SwissProt (Rat)
Q6DGG0
CAS Number
9007-83-4
Available Sizes
Curated Selection
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