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Rabbit anti-Caspase 3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Caspase 3

Synonyms

CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Host

Rabbit

Reactivity

H

Immunogen

Recombinant protein corresponding to human Caspase 3.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Caspase 3 expression in Hela (A) whole cell lysates.

IHC description

Immunohistochemical analysis of Caspase 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

836

SwissProt (Human)

P42574

CAS Number

9007-83-4

Available Sizes

Curated Selection

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