Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)(PC5), CF405S conjugate
Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Specifications
Synonyms
Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
PCNA
Gene ID
5111
NCBI Gene ID
147433 & 728886
UniProt
P12004
Cellular Locus
Nucleus & cytoplasm
Host
Mouse
Species Reactivity
Human,Mouse,Rat,Yeast
Immunogen
Rat PCNA/Protein A fusion protein
Target Antigen
PCNA
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
PC5
Conjugation
CF405S
Source
Animal
Applications
IHC, FFPE (verified) | WB (verified)
Validated Applications
IHC,FFPE,WB
Positive Control
Tonsil or reactive lymph node. MOLT-4, HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937.
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
36 kDa
Shipping Conditions
Room temperature
Storage Conditions
4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
2 years
Product Datasheet
https://cdn.gentaur.com/products/37/7647889/datasheet/bnc043065-100.pdf
Product MSDS
https://cdn.gentaur.com/products/37/7647889/msds/bnc043065-100.pdf
CAS Number
9007-83-4
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