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Anti-CD29 (Stem Cell Marker)(12G10), CF405S conjugate

Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

Fibrinogen Receptor beta subunit; FNRB; Glycoprotein IIa (GPIIA); Integrin beta-1 (ITGB1); integrin VLA-4 beta subunit; MDF2; MSK12; Very Late Activation Protein beta Polypeptide; VLA-4 subunit beta; VLA-BETA; VLAB

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ITGB1

Gene ID

3688

NCBI Gene ID

643813

UniProt

P05556

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human

Immunogen

Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract.

Target Antigen

CD29

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

12G10

Conjugation

CF405S

Source

Animal

Applications

Flow, surface (published) | Functional studies (published) | IF (published) | IP (published)

Validated Applications

FC,IF,IP

Positive Control

HT1080 whole cell lysate. HeLa cell line.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

110 kDa (non-reduced) & 130 kDa (reduced)

Additionnal Information

Does not react with Mouse or Rat; ELISA: For coating use Ab at 1-5 ug/mL, order Ab without BSA; Optimal dilution for a specific application should be determined by user.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. FEBS Lett (1995) 363: 118-122. (functional studies) J Cell Sci (2005) 118: 4009-4016. (IP) Traffic (2012) 13: 610-625. (Flow, IF)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/7646544/datasheet/bnc042905-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/7646544/msds/bnc042905-500.pdf

CAS Number

9007-83-4

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