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Anti-WIPI2(2A2)

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al. , 2004 ). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WIPI2; WIPI49 like protein 2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

WIPI2

Gene ID

26100

NCBI Gene ID

122363

UniProt

Q9Y4P8

Cellular Locus

Plasma membrane

Host

Mouse

Species Reactivity

Human,Mouse

Immunogen

Recombinant fragment (around aa 418-436) of human WIPI2 protein (exact sequence is proprietary)

Target Antigen

WIPI2

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

2A2

Conjugation

Purified, BSA-free

Source

Animal

Applications

IF (published) | IP (published) | WB (published)

Validated Applications

IF,IP,WB

Positive Control

HEK293 cells. Human or mouse placenta, skeletal muscle or testis.

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

49 kDa

Additionnal Information

ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Autophagy (2010) 6(4):506-522. (IF) Brain (2019) 142(5): 1242-1254. (IF, IP, WB)

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/7646542/datasheet/bnum2904-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/7646542/msds/bnum2904-50.pdf

CAS Number

9007-83-4

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