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Anti-CLEC9A(1F6), CF640R conjugate

This Mab recognizes Mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a and the CD103 DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31( ) dendritic cells and on a small subset of CD14( ) and CD16(-) monocytes._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

C type lectin domain family 9; member A; C-type lectin domain family 8 member A; CLEC9A; DNGR1; HEEE9341; PRO34046; UNQ9341; CD370

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

232414

NCBI Gene ID

391518

UniProt

Q8RU4

Cellular Locus

Plasma membrane

Host

Rat

Species Reactivity

Mouse

Immunogen

RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.

Target Antigen

CLEC9A

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1F6

Conjugation

CF640R

Source

Animal

Applications

Flow, surface (published) | Functional studies (published)

Validated Applications

FC,Functional assays

Positive Control

Mouse spleen.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

27 kDa

Additionnal Information

Flow cytometry: 0.5-1 ug/million cells; Western blot 0.5-1 ug/mL; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Nature (2009) 458(7240): 899-903. (functional studies) J Immunol (2015) 195:5066-5076. (Flow, surface)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/7639237/datasheet/bnc402209-500.pdf

Product MSDS

https://cdn.gentaur.com/products/37/7639237/msds/bnc402209-500.pdf

CAS Number

9007-83-4

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