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Anti-CD8b, Mouse(H35-17.2), CF488A conjugate

This rat monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Specifications

Synonyms

CD8b antigen; CD8 antigen beta polypeptide 1 (p37); CD8 beta; CD8B1; Leu2; Ly3; LYT3; P37; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

Cd8b1 (Mouse)

Gene ID

12526

NCBI Gene ID

3331

UniProt

P10300

Cellular Locus

Plasma membrane

Host

Rat

Species Reactivity

Mouse

Immunogen

Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

Target Antigen

CD8b,

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

H35-17.2

Conjugation

CF488A

Source

Animal

Applications

Flow, surface (published) | Functional studies (published)

Validated Applications

FC,Functional assays

Positive Control

MPBMC's or Thymus.

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

37 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Infection Immunity (1994) 62(5): 1820-1829. (functional studies) Meth Mol Biol vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_2 (Flow, surface)

Shipping Conditions

Room temperature

Storage Conditions

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/7637067/datasheet/bnc882003-100.pdf

Product MSDS

https://cdn.gentaur.com/products/37/7637067/msds/bnc882003-100.pdf

CAS Number

9007-83-4

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