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HEK293 Host Cell DNA (HEK293 HCD) Residue Detection kit

Purchasing this kit includes a CG-DP050 complimentary Magnetic Residual DNA Sample Preparation Kit-50T, designed for DNA extraction.

Product Specifications

Reproducibility

1) Intra Variation: 10.3 - 12.3 % || 2) Inter Variation: 16 - 19 %

Certification

ISO 13485:2016

Species Reactivity

Human

Target

HEK 293 Host Cell DNA Residue

Applications

PCR

Field of Research

Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell.

Assay Type

PCR

Detection Range

30 fg/μL - 3000 pg/μL, the detection range can also be adjusted.

Precision

1) Intra Variation%: 10.3-12.3 2) Inter Variation%: 16-19

Sensitivity

LOQ: 30fg/μL

Components

(1) 2X qPCR Mix (UDG plus) || (2) Primer&Probe Mix || (3) DNA Dilution Buffer || (4) DNA Control (30ng/μL) || (5) RNase-Free H2O || (6) 50X ROX Reference Dye (Optional)

Storage Conditions

The kit should be stored at -20°C. The shelf life of components B2/B3/B4 is 2 years. The shelf life of components B1/B5/B6 is 1 year.

Species

HEK 293

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