Rabbit anti-ASK1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ASK1
Synonyms
ASK1; MAPKKK5; MEKK5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ASK1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ASK1 expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of ASK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4217
Gene ID (Mouse)
26408
SwissProt (Human)
Q99683
SwissProt (Mouse)
O35099
CAS Number
9007-83-4
Available Sizes
Curated Selection
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