CKB Antibody / CKBB / Creatine kinase B

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: for coating order antibody without BSA, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P12277

Host

Mouse

Reactivity

Human, Mouse, Rat, Hamster, Guinea pig

Immunogen

A recombinant human full-length protein was used as the immunogen for the CKB antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CKBB/6565

Applications

ELISA, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CKB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CKB antibody is available for research use only.

Storage Conditions

Aliquot the CKB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CKB antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human kidney tissue with CKB antibody (clone CKBB/6565) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.