RAD50 Antibody

DNA repair protein RAD50, also known as RAD50, is a protein that in humans is encoded by the RAD50 gene. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q92878

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids N1224-H1312) was used as the immunogen for the RAD50 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the RAD50 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RAD50 antibody is available for research use only.

Storage Conditions

After reconstitution, the RAD50 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RAD50 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human A549 cells with RAD50 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.