PDGF-BB Recombinant
Recombinant PDGF-BB is a disulfide-linked homodimeric protein consisting of a two 109 amino acid residue subunits, and migrates as an approximately 32 kDa protein under non-reducing and 16 kDa under reducing conditions. Optimized DNA sequence encoding human PDGF-BB mature chain was expressed inSaccharomyces cerevisiaecells.
Product Specifications
UniProt
P01127
Endotoxin
Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Purity
≥ 98% by SDS-PAGE and HPLC analysis.
Format
Lyophilized protein
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.
Shipping Conditions
-20°C or-80°C
Storage Conditions
≥2 years (lyophilized) at -20°C.
Formulation
Lyophilized from a 0.2 µm filtered 0.02M acetic acid solution, pH 6.5.
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