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Biotin / Vitamin B7 / Vitamin H Antibody

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Specifications

UniProt

Not Applicable

Host

Mouse

Immunogen

Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.

Clonality

Monoclonal

Isotype

IgG1 κ

Applications

FACS, IF, WB, IHC-P

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Biotin antibody is available for research use only.

CAS Number

9007-83-4

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