Smooth Muscle Actin Antibody

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells.</strong> This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P62736

Host

Mouse

Reactivity

Human

Immunogen

N-Terminal decapeptide of alpha smooth muscle isoform of actin was used as the immunogen for the Smooth Muscle Actin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RACTA2/1625

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Smooth Muscle Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Smooth Muscle Actin antibody is available for research use only.

Storage Conditions

Aliquot the Smooth Muscle Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human uterus tissue with Smooth Muscle Actin (clone rACTA2/1625) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.