Alpha Fetoprotein Antibody
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP) . This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P02771
Host
Mouse
Reactivity
Human
Immunogen
Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM334
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Alpha Fetoprotein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Alpha Fetoprotein antibody is available for research use only.
Storage Conditions
Store the Alpha Fetoprotein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/alpha-fetoprotein-antibody-spm334-v9012
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human fetal liver stained with AFP antibody (SPM334) .
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