Biotin / Vitamin B7 / Vitamin H Antibody
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Product Specifications
UniProt
Not Applicable
Host
Mouse
Immunogen
Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
Clonality
Monoclonal
Isotype
IgG1 κ
Applications
FACS, IF, WB, IHC-P
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Biotin antibody is available for research use only.
CAS Number
9007-83-4
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