Histone H3 Recombinant Antibody (pSer10)
Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
Product Specifications
UniProt
P68431
Host
Mouse
Immunogen
A synthetic peptide corresponding to the amino acids surrounding phospho S10 were used as the immunogen for the recombinant PHH3 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Type
Recombinant
Applications
IHC-P
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant PHH3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant PHH3 antibody is available for research use only.
CAS Number
9000-83-3
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