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RPA32 Antibody / Replication Protein A2

Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.

Product Specifications

Host

Mouse

Immunogen

A recombinant human full-length protein was used as the immunogen for this Replication Protein A2 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Applications

WB

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Replication Protein A2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Replication Protein A2 antibody is available for research use only.

CAS Number

9007-83-4

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