CD20 Antibody

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P11836

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 213-297 from the human protein were used as the immunogen for this CD20 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MS4A1/3409

Applications

ELISA, FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This CD20 antibody is available for research use only.

Storage Conditions

CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Predominantly cell surface with some cytoplasmic

Image Legend

IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/3409) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.