Synaptophysin Antibody

This monospecific monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08247

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human Synaptophysin protein fragment within amino acids 224-313 was used as the immunogen for the Synaptophysin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SYP/3551

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Synaptophysin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Synaptophysin antibody is available for research use only.

Storage Conditions

Store the Synaptophysin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human pancreatic carcinoma with Synaptophysin antibody (clone SYP/3551) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.