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CD73 Antibody

The protein encoded by the NT5E gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order BSA-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P21589

Host

Mouse

Reactivity

Human

Immunogen

A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDLA73-1

Applications

IHC-P, IF, WB, FACS, ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD73 antibody is available for research use only.

Storage Conditions

Store the CD73 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone CDLA73-1) ; Red=isotype control, Blue= CD73 antibody.

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