PAPPA Antibody / Pappalysin-1

The PAPPA gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs) . Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

Q13219

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 351-523 from the human protein was used as the immunogen for the PAPPA antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

IDIP-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PAPPA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PAPPA antibody is available for research use only.

Storage Conditions

Store the PAPPA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human placenta with PAPPA antibody (clone IDIP-1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.