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Recombinant SPTA1 Antibody / Spectrin alpha 1

This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P02549

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 356-475 from the human protein was used as the immunogen for the recombinant SPTA1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

SCNA-1R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant SPTA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant SPTA1 antibody is available for research use only.

Storage Conditions

Store the recombinant SPTA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, cell surface

Image Legend

Western blot testing of human Hela cell lysate with recombinant SPTA1 antibody (clone SCNA-1R) . Expected molecular weight: 260-280 kDa.

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