NSE Antibody
Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
UniProt
P09104
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
NSEL1-1
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the NSE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NSE antibody is available for research use only.
Storage Conditions
Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/nse-antibody-nsel1-1-v7210
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic
Image Legend
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