Ku70 + Ku80 Antibody

Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D) J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. In addition to its role in NHEJ, Ku is also required for telomere length maintenance and subtelomeric gene silencing. Ku was originally identified when patients with systemic lupus erythematosus were found to have high levels of autoantibodies to the protein. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

A human cell nuclear fraction was used as the immunogen for this Ku70 + Ku80 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LKAP1-1

Applications

FACS, IF

Purity

Protein G purified

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Ku70 + Ku80 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Ku70 + Ku80 antibody is available for research use only.

Storage Conditions

Store the Ku70 + Ku80 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the Ku70 + Ku80 antibody may be required for optimal performance.

Location

Nuclear

Image Legend

Immunofluorescent staining of paraformaldehyde fixed human HeLa cells with Ku70 + Ku80 antibody (clone LKAP1-1, green) and Phalloidin cell membrane stain (red) .