Smooth Muscle Actin Antibody

Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P62736

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SMAT2-1

Applications

IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Smooth Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Smooth Muscle Actin antibody is available for research use only.

Storage Conditions

Store the Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human angiosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.