EMI1 Antibody (Early Mitotic Inhibitor-1)
Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Product Specifications
UniProt
Q9UKT4
Host
Mouse
Immunogen
A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Applications
WB, IHC-P
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the EMI-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EMI-1 antibody is available for research use only.
Product Datasheet
https://cdn.gentaur.com/products/800/6699421/datasheet/v7090-20ug.pdf
CAS Number
9007-83-4
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