TTF-1 Antibody / NKX2.1

Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P43699

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant protein was used as the immunogen for the TTF-1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HBNK2-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TTF-1 antibody is available for research use only.

Storage Conditions

Store the TTF-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titering of the TTF-1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Nuclear

Image Legend

IHC testing of FFPE human thyroid tissue with TTF-1 antibody (clone HBNK2-1) . Required HIER: boil FFPE tissue sections in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.