AURKB Antibody / Aurora B

Aurora B is a serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q96GD4

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 89-251 were used as the immunogen for this AURKB antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

ARRK2-1

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the AURKB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This AURKB antibody is available for research use only.

Storage Conditions

Store the AURKB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the AURKB antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human prostate tissue with AURKB antibody (clone ARRK2-1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.