Mammaglobin Antibody / SCGB2A2

Mammaglobin, also called Secretoglobin family 2A member 2, or SCGB2A2, is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13296

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the Mammaglobin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MGB/2000

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Mammaglobin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Mammaglobin antibody is available for research use only.

Storage Conditions

Store the Mammaglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Mammaglobin antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface, nuclear

Image Legend

IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB/2000) . Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.