Recombinant Multi Cytokeratin Antibody

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) ; 56.5kDa (CK10) ; 53kDa (CK13) and 45kDa (CK18) . This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Multi

Host

Mouse

Reactivity

Human

Immunogen

A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKRT/457

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Multi Cytokeratin antibody is available for research use only.

Storage Conditions

Store the recombinant Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human skin with recombinant Multi Cytokeratin antibody (clone rKRT/457) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.