CD22 Antibody

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P20273

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 52-178 from the human protein were used as the immunogen for the CD22 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BLCAM/1795

Applications

ELISA, FACS, WB, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD22 antibody is available for research use only.

Storage Conditions

Store the CD22 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD22 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human tonsil tissue with CD22 antibody (clone BLCAM/1795) . HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.