Recombinant p75NTR Antibody / NGFR

This antibody is specific for a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or p75 Neurotrophin Receptor (p75NTR) . NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR/p75NTR is a reliable marker for desmoplastic and neurotropic melanomas. It is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR/p75NTR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08138

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant p75NTR antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

NGFR/1997R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant p75NTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant p75NTR antibody is available for research use only.

Storage Conditions

Store the recombinant p75NTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant p75NTR antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cell surface and cytoplasmic

Image Legend

IHC testing of FFPE human melanoma tissue with recombinant p75NTR antibody. Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.