P53 Antibody
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P04637
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human p53 protein was used as the immunogen for this p53 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
FACS, IF, WB, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p53 antibody is available for research use only.
Storage Conditions
Store the p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/p53-antibody-v3343
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the p53 antibody for each application should be determined by the researcher.
Location
Predominantly nuclear
Image Legend
IHC testing of FFPE human colon carcinoma with p53 antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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