Filaggrin Antibody

Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P20930

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 198-288 of human FLG were used as the immunogen for this Filaggrin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

FLG/1561

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Filaggrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Filaggrin antibody is available for research use only.

Storage Conditions

Store the Filaggrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Filaggrin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin with Filaggrin antibody (clone FLG/1561) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.