CD99 Antibody
CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 5-10 µg/mL for 30 min at RT
UniProt
P14209
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the CD99 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD99 antibody is available for research use only.
Storage Conditions
Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the CD99 antibody may be required for optimal performance.
Location
Cell surface
Image Legend
IHC testing of FFPE human Ewing's sarcoma with CD99 antibody. Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
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