Beta-2 Microglobulin Antibody
Recognizes a protein of 12kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha- heavy chain that contains three subdomains (alpha-1, alpha-2, alpha-3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha-3 subdomain of the alpha- heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha-1 and alpha-2 domains of the alpha- heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P61769
Host
Mouse
Reactivity
Human
Immunogen
MOLT-4 human T cell line was used as the immunogen for the Beta-2 Microglobulin antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
BBM.1
Applications
FACS, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Beta-2 Microglobulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Beta-2 Microglobulin antibody is available for research use only.
Storage Conditions
Store the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of permeabilized human HeLa cells with Beta-2 Microglobulin antibody (green, clone BBM.1) and Reddot nuclear stain (red) .
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